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实验参考方案:
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
产品描述:
Phosphatase Inhibitor Cocktail II effectively inhibits acid and alkaline phosphatases as well as protein tyrosine phosphatases(PTPs).
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents. Provided as 1 ml vial containing the following components: Imidazole (200 mM), Sodium Fluoride (100 mM), Sodium Molybdate (115 mM), Sodium Orthovanadate (100 mM), and Sodium Tartrate Dihydrate (400 mM).
Phosphatase Inhibitor Cocktail II has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in ddH2O.
Components
| Ingredient | M.W. | 100x Conc. | Target |
| Sodium Fluoride | 42.0 | 100 mM | Acid phosphatases |
| Sodium Orthovanadate | 183.9 | 100 mM | Alkaline phosphatases, PTPs, ATPases |
| Sodium Tartrate Dihydrate | 230.1 | 400 mM | Acid phosphatases |
| Sodium Molybdate | 205.9 | 115 mM | Acid and phosphoprotein phosphatases |
| Imidazole | 68.1 | 200 mM | Alkaline phosphatases |
