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实验参考方案:
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
产品描述:
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Each vial contains a 1 ml solution with the following components: 500 µM Cantharidin, 2.5 mM (-)-p-Bromotetramisole oxalate, and 1 µM Calyculin A form Discodermia calyx.
Phosphatase Inhibitor Cocktail III has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.
Components
| Ingredient | M.W. | 100x Conc. | Target |
| (-)-p-Bromolevamisole oxalate | 373.2 | 2.5 mM | Alkaline phosphatases |
| Cantharidin | 196.2 | 500 µM | protein phosphatase 2A(PP2A) |
| Calyculin A | 1009.2 | 1 µM | protein phosphatases 1 and 2A(PP1 and PP2A) |
