GK20006-0.75ml / Lipo3000 Transfection Reagent
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￥2000.00
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Preparation Before Experiment
• Plasmid DNA (0.5–5 μg/μL stock solution)
• Opti-MEM reduced serum medium
• Microcentrifuge tube
Experimental Steps
A. Super Lipo3000 DNA Transfection Reagent requirements
| Component | 96-well | 24-well | 6-well |
| Final DNA per well | 100 ng | 500 ng | 2500 ng |
| Final Lipo3000 Reagent per well | 0.2–0.5 μL | 1.0–2.5 μL | 5.0–12.5 μL |
| Final P-3000 Reagent per well | 0.2 μL | 1.0 μL | 5.0 μL |
B. Transfection of siRNA
When transfecting siRNA into cells, follow the DNA protocol described above, but do not add P-3000 when diluting siRNA (Step 3)
C. SuperLipo3000DNA Transfection Reagent Protocol
Transfect the cells according to the table below, using the specified volume of DNA and P-3000 reagent and the corresponding two volumes of Lipo3000 (when optimized). The volume of each reaction mixture is the volume of a single well, and the pipetting difference is considered. Calculate the volume of other holes in proportion.
品牌
GLPBIO
产品编号
GK20006
规格
0.75ML
货期
现货
