线上账号

货号：EXO-FBS-50A-1

规格：50ml

保存条件：-20

运输条件：干冰

用途：适用于细胞研究、外泌体研究

特点：无支原体、无内毒素

库存状态：原装，常备现货

特惠价格：询价（量大折优）

温馨提示：该产品不可用于临床治疗

产品货号：EXO-FBS-50A-1   

    zui近的研究发现外泌体在很多生理病理上起着重要的作用，这些发现点燃了人们对细胞分泌膜泡的兴趣。exosome及其他细胞外囊泡正起着不可缺少的细胞通讯作用。在肿瘤发展过程中，exosome介导了关键的步骤，如刺激血管生成，削弱免疫反应，甚至参与了转移前微环境的形成。在正常的生理过程中，exosome也起了重要作用，如胎盘exosome帮助形成对母亲免疫系统的免疫抑制屏障。总之，在正常和病理的条件下，细胞都释放小囊泡，以各种方式影响其他细胞，外泌体特指直径在30-100nm的盘状囊泡，其内部包裹了多种蛋白质、mRNA、non-coding RNA等分子。外泌体主要来源于细胞内多囊泡体，经多囊泡体外膜与细胞膜融合后释放到胞外基质中。目前大量文献报道，几乎所有类型的细胞都可以产生并释放外泌体，不同的生物体液(血液、尿液、脑脊液、唾液、乳汁、腹水)中都发现有大量外泌体存在，外泌体由细胞分泌释放后，通过个种体液在体内传播，被靶细胞摄入后，释放内部的生物分子，参与细胞间的通讯，调节细胞功能。

SBI- EXO-FBS-50A-1无外泌体血清 /SBI原装进口   

Exo-FBS Overview: When You're Not Studying Bovine Exosomes

   Do you study bovine exosomes? If not, and you use fetal bovine serum (FBS) in your cell or tissue culture media, you may be isolating more bovine exosomes than you realize. Serum is a rich source of exosomes, FBS included. In order to help scientists study only the exosomes they want, SBI provides Exo-FBS™ exosome-depleted FBS.

·         Exosome-sized vesicles removed

·         Very low levels of CD63 positive cow exosome

·         Undetectable levels of cow microRNAs

·         Comparable growth rates as standard FBS

·         Identical use as standard FBS (add 10% in DMEM or RPMI)  

Background

Standard growth media for most cells in culture require FBS as a growth supplement to DMEM. FBS is derived from bovine (cow) serum and contains a high abundance of cow exosome vesicles. These exosomes can interfere or cause significant background issues when studying the exosomes secreted from your cells of interest in standard culture conditions.

SBI has developed an exosome-depleted FBS growth supplement called Exo-FBS that has been stripped of bovine exosomes. Exo-FBS supports equivalent growth of many types of cells in culture, is devoid of cow CD63-positive exosomes, and does not have any measurable bovine microRNAs. Cell growth in Exo-FBS is comparable to standard FBS. The process of manufacturing of Exo-FBS is a patented method in Patent No.: US 9,005,888 B2.

Conclusions

Perform your studies of cellular secreted exosomes in culture without the worry of contaminating cow exosomes in your experiments, and with no ultracentrifugation required when you use ExoQuick-TC. Available as standard FBS supplement or heat inactivated FBS media supplement (treated at 65°C for 15 minutes before bovine exosome removal).